Promoter Methylation Status and Expression Levels of RASSF1A Gene in Different Phases of Acute Lymphoblastic Leukemia (ALL)

Background: Although the precise pathogenesis of acute lymphoblastic leukemia (ALL) remains unclear, studying gene-regulating mechanisms during ALL pathogeneses may shed light on underlying driving malignant behavior. There is some evidence showing promoter hypermethylation and silencing RASSF1A tumor suppressor gene in cells; however, there a lack for whether indeed alters different phases or response to therapy. Thus, current study aimed clarify this issue using groups adult patients who have been scarcely investigated regarding expression levels methylation status.
 Materials Methods: In case/control study, status was evaluated quantitative real-time PCR methylation-specific (MSP), respectively adults with ALL. The included peripheral blood newly diagnosed (n=10), complete remission (CR) relapse 10 control samples from healthy individuals.
 Results: MSP results revealed an unmethylated almost all samples, except case relapsing ALL, which showed hemimethylated pattern. also no difference terms compared group (p>0.05).
 Conclusion: up-regulation experiencing CR, suggesting be marker therapy response. However, further investigations more sensitive detecting tools larger sample sizes better involvement these patients.